Subfractions of enamel matrix derivative differentially influence cytokine secretion from human oral fibroblasts
نویسندگان
چکیده
منابع مشابه
Subfractions of enamel matrix derivative differentially influence cytokine secretion from human oral fibroblasts
Enamel matrix derivative is used to promote periodontal regeneration during the corrective phase of the treatment of periodontal defects. Our main goal was to analyze the bioactivity of different molecular weight fractions of enamel matrix derivative. Enamel matrix derivative, a complex mixture of proteins, was separated into 13 fractions using size-exclusion chromatography and characterized by...
متن کاملCellular viability and genetic expression of human gingival fibroblasts to zirconia with enamel matrix derivative (Emdogain®)
PURPOSE The objective of this study was to investigate the biologic effects of enamel matrix derivative (EMD) with different concentrations on cell viability and the genetic expression of human gingival fibroblasts (HGF) to zirconia surfaces. MATERIALS AND METHODS Immortalized human gingival fibroblasts (HGF) were cultured (1) without EMD, (2) with EMD 25 µg/mL, and (3) with EMD 100 µg/mL on ...
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Emdogain, a formulation of Enamel Matrix Proteins, is used clinically for periodontal regeneration to stimulate PDL (periodontal ligament), cementum, and bone formation. Its effects on gingival fibroblasts and tissue have not been thoroughly studied. Therefore, we investigated the mechanisms by which Emdogain affects the cell cycle of human gingival fibroblasts. Without serum, Emdogain (50 micr...
متن کاملThe Use of Enamel Matrix Derivative
Background—Periodontal disease results in the loss of the attachment apparatus. In the last three decades, an increasing effort has been placed on seeking procedures and materials to promote the regeneration of this tissue. The aim of this paper is to evaluate the effect of enamel matrix derivative (EMD) during regenerative procedures. In addition, a meta-analysis is presented regarding the cli...
متن کاملRoot coverage technique with enamel matrix derivative.
Various periodontal plastic surgical techniques are employed in obtaining root coverage. Recently, the use of an enamel matrix derivative (EMD) has been reported in such treatment. We report 2 cases of root coverage surgery with a coronally positioned flap in combination with EMD (CPF+EMD) and connective tissue graft in combination with EMD (CTG+EMD). Case 1: The patient was a 25-year-old woman...
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ژورنال
عنوان ژورنال: Journal of Tissue Engineering
سال: 2015
ISSN: 2041-7314,2041-7314
DOI: 10.1177/2041731415575857